siRNA PK/TK/PD analysis

본문 바로가기

siRNA PK/TK/PD analysis

Modified stem-loop RT-qPCR quantification
  • Selection of optimal primers and probes using a minimum of 8 designs
  • Establishment of conditions for cDNA synthesis and stem-loop RT-qPCR through repeat tests
Quantitative method validation and optimization
  • siRNA quantitative method validation (in triplicate) and optimization on naked and formulated siRNA
  • Standard and amplification curves
  • Verification of siRNA quantitation in plasma
Pharmacokinetics analysis from samples of non-clinical & clinical trials
  • Sample tests
Validation of siRNA-directed mRNA cleavage
  • siRNA specific primer design
  • 5'-RACE-PCR