LNP for mRNA based vaccine, siRNA therapeutics,
Therapeutic protein replacement, Genome editing and Cell engineering
EN Lipid Nano Particle for mRNA & RNA therapeutics
Lipid Nanoparticles (LNPs) are the most clinically advanced non-viral gene delivery system. It safely and effectively delivers nucleic acids, overcoming a major barrier preventing the development and use of genetic medicines.
Lipid nanoparticles offer many advantages over previous lipid-based nucleic acid delivery systems including:- High nucleic acid encapsulation efficiency and potent transfection
- Improved penetration into tissues to deliver therapeutics
- Low cytotoxicity and immunogenicity
EN-LNP: Structure
EN-LNP: Structure
Ionizable Cationic Lipid (246C10)
- High Encapsulation efficiency
- LNP structure and stabilization
- Nucleic acid Encapsulation
- Endosomal disruption
- Responsible for payload release
Nucleic acid
- DNA, mRNA, small RNA etc.
Cholesterol
- Mimics LDL -> Uptake
- LNP stabilization
Cholesterol
- Helper lipid
PEG-lipid
- Stabilize in systemic circulation
- LNP protection (Shield (stealth) effect)
- from nonspecific endocytosis by immune cells
- reduces LNP aggregation and shields the LNP
Electron microscope image of EN-LNPs
- M.Kim et al., Engineered ionizable lipid nanoparticles for targeted delivery of RNA therapeutics into different types of cells in the liver. Sci. Adv. 7 eabf4398 (2021).
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